Regulatory

Part:BBa_K2997004:Design

Designed by: Ting-Han Sung   Group: iGEM19_NCKU_Tainan   (2019-10-12)


PchR Protein Binding Range


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Eventually, the positive P. fluorescens 55 clone with our pchR-GFP construct is chosen to undergo the functional test as P. aeruginosa DAP is a BSL2 classified organism, which is not aligned with our aim of having a safe detection device.

The functional test results are depicted below:



Source

Eventually, the positive P. fluorescens 55 clone with our pchR-GFP construct is chosen to undergo the functional test as P. aeruginosa DAP is a BSL2 classified organism, which is not aligned with our aim of having a safe detection device.

The functional test results are depicted below:


References